About the project
Project Manager
Zhumagul Slyambekovna Kirkimbaeva
Doctor of Veterinary Sciences, Professor
Scopus Author ID: 56273059600
Researcher ID: FCY-1524-2022
ORCID: https://orcid.org/0000-0001-8820-9260
Relevance
Fisheries play an important role in the economy of the Republic of Kazakhstan and are one of the most promising and rapidly developing sectors of the national economy. According to the Fisheries Committee of the Ministry of Agriculture of the Republic of Kazakhstan, by 2027 the number of fish farms will increase to 700, i.e. 25% more than in 2025, and the volume of fish farming is planned to reach 50 thousand tonnes. Despite the positive dynamics in aquaculture production, there are problems that hinder the growth and development of this industry, including bacterial infections. The dominance of pathogens against the background of a weakening of the macroorganism's defences can lead to the onset of an epizootic. The use of antibiotics to stabilise the epizootic situation, as well as the use of antibiotic mixtures in feed as a growth stimulant, leads to the development of multi-resistance to antibiotics in strains of pathogenic bacteria, horizontal transfer of resistance to the bacterial community and a decrease in the effectiveness of antibiotic treatment of human and animal diseases. The World Health Organisation (WHO) has noted that resistance to antimicrobial drugs is a serious threat to global health and food security. In this regard, the development of alternative methods for the prevention and treatment of bacterial diseases based on a multidisciplinary approach using methods to correct the intestinal microbiota of fish in aquaculture conditions is highly relevant. The study of the fish gut microbiome provides insight into the species composition of the microbiota, allows analysis of the state of the fish organism and its immune system, and enables measures to be taken to preserve and create an optimal microflora composition by adjusting the diet and using alternative methods of prevention and treatment to antibiotics.
Purpose
To study the species diversity, phenotypic and genotypic features of pathogens causing bacterial infections in fish and to develop methods for preventing bacterioses in aquaculture
Expected and achieved results
The implementation of the project will yield the following results:
- Monitoring studies will be conducted to identify the etiological structure of bacterial infections of fish in aquaculture conditions.
- Communities of the intestinal microbiome of fish will be studied by conducting microbiological analysis and genotyping, taking into account age and seasonal aspects.
- The effect of the feed used and the bacteriological parameters of water on the composition of the intestinal microflora of fish will be studied.
- Morphological, cultural, physiological, biochemical and probiotic properties of Lactobacillus plantarum DLC 12-47 and Lactobacillus paracasei DDL-3-16 strains will be studied.
- A technology will be developed to produce a new probiotic drug based on Lactobacillus plantarum DLC 12-47 and Lactobacillus paracasei DDL-3-16 strains to correct the intestinal microbiome of aquaculture.
- The effect of the probiotic drug on the indicators of natural resistance of the fish body, the composition of the intestinal microbiome and the morphology of the internal organs of fish will be studied.
- Pilot industrial samples of a probiotic drug based on a consortium of Lactobacillus plantarum DLC 12-47 and Lactobacillus paracasei DDL-3-16 strains will be created to correct the intestinal microbiome of aquaculture.
- The optimal dose of probiotic drug injection into fish feeds of different ages will be determined;
- A veterinary and sanitary assessment of the quality of fish meat in terms of safety and nutritional value will be given when using a new probiotic. The current results of the project will be implemented by obtaining patents for a utility model and publishing articles in foreign peer-reviewed scientific journals.
Information for potential users
Development of probiotic preparations from Lactobacillus plantarum DLC 12-47 and Lactobacillus paracasei DDL-3-16 based on the study of antagonistic activity against the main causative agents of fish bacteriosis and adaptation for their introduction into aquaculture as preparations aimed at correcting the intestinal microbiome can be effectively used to increase the resistance of fish to bacterial infections in the conditions of fish farms in Kazakhstan. This will reduce morbidity and mortality in aquaculture and reduce the cost of veterinary measures. The results obtained create an opportunity to develop a line of probiotic preparations based on previously obtained results, including probiotics based on the Lactobacillus consortium and the probiotic preparation E.coli. There will be opportunities for cooperation with manufacturers of veterinary drugs or for the creation of a production laboratory at the university. The developed probiotic can be commercialised and introduced to the veterinary drug market in Kazakhstan.
Project team
1. Sarsembaeva Nurzhan
Doctor of Veterinary Sciences, Professor
Scopus Author ID: 55928669100
Researcher ID: FXP-2929-2022
ORCID: https://orcid.org/0000-0002-3501-3720
2. Ermagambetova Svetlana
candidate of Veterinary Sciences
Scopus Author ID: 57210559053
Researcher ID: ORCID: https://orcid.org/0000-0003-0426-2561
3. Sarybayeva Dinara
PhD
Scopus Author ID: 56271818700
Researcher ID: FUF-1064-2022
ORCID: https://orcid.org/0000-0001-7081-1632
4. Boranbayeva Togzhan
PhD
Scopus Author ID: 56072778500
Researcher ID: GZG-8772-2022
ORCID: https://orcid.org/0000-0002-1159-1200
5. Zhylkaidar Arman
PhD
Scopus Author ID: 57212588141
ORCID: https://orcid.org/0000-0003-2439-9792
6. Kenzhegalieva Meruyert
master's degree
ORCID: https://orcid.org/0009-0001-0427-6675
7. Yessentureyeva Meruyert
master's degree
Scopus Author ID:
Researcher ID: https://www.researchgate.net/profile/Meruyert-Yessentureyeva?ev=hdr_xprf
ORCID: https://orcid.org/0000-0002-4354-5145