About the project
Project Manager
Beloussov Vyacheslav
Candidate of biological sciences
Scopus Author ID: 58030218400
Researcher ID: AEK-7910-2022
ORCID: https://orcid.org/0000-0003-1922-156X
Relevance
WHO calls for global action to combat sepsis. The first WHO global report on sepsis concludes that efforts to combat sepsis are hampered by serious gaps in scientific and clinical knowledge, particularly in low- and middle-income countries, including Kazakhstan.
The basis of sepsis pathogenesis is still not completely clear, and includes complex systemic inflammatory network effects, gene polymorphism, immune dysfunction, abnormal blood coagulation, tissue damage and abnormal host responses to various infectious pathogens and their toxins. This fact complicates the search for informative biomarkers, the number of which currently numbers in the tens and hundreds.
Project objective
The comparative analysis of the transcriptome for biomarkers and endotypes of sepsis by high-throughput sequencing (NGS RNA-seq). As a result of the project, the transcriptome will be analyzed simultaneously in blood and urine samples, in groups of sepsis patients and conditionally healthy individuals, taking into account variants of disease severity and sepsis outcomes, including isolation, identification and antimicrobial susceptibility of the pathogen.
Expected and achieved results
It is expected that transcriptome analysis data, based on the expression of more than 20,800 human genes included in the RefSeq database will allow us to detect differences in the expression profiles of the studied groups, as well as to confirm the presence of characteristic sepsis endotypes - predictors of the risk of complications and mortality described in the literature. The differential gene expression data (DEG) and endotypes thus obtained will generally help to improve the understanding of molecular and cellular processes in sepsis, and will also allow the future use of these data for the selection of specific biomarkers, with the aim of subsequently creating and utilizing a diagnostic test system. Successful completion of this project will stimulate wider implementation and development of transcriptomic methods in various fields of not only medicine, but also related fields of natural sciences in the Kazakhstan, as well as serve as a good basis for training of specialists in this field.
Information for potential users
The scientific novelty and significance of the project lies in the fact that for the first time a comparative analysis of the transcriptome by NGS in venous blood and urine samples from sepsis patients and control group of conditionally healthy patients will be carried out, including identification of the pathogen, its resistance to antimicrobial drugs; analysis of DEG in the studied samples and groups; analysis of expression correlation, with data on pathogen identification and antimicrobial susceptibility, severity and outcome of the disease, as well as comparison of DEG results and sepsis endotypes with available literature data. The use of molecular genetic diagnostic methods based on NGS RNA-seq will lead not only to a better understanding of the processes of sepsis onset and course, associated with the severity of the disease and its outcomes, but will also make it possible to use the obtained data for practical purposes, to create a rapid, accurate and not relatively inexpensive diagnostic and predictive test system based on PCR.
Members of the research group
1. Sandybayev N.
PhD, professor
Scopus Author ID: 37000362400
Researcher ID: AAO-4831-2021, M-7802-2015
ORCID: http://orcid.org/0000-0003-1814-2798
2. Lavrinenko A.
PhD
Scopus Author ID: 57193763117
https://orcid.org/0000-0001-9436-8778
3. Strochkov V.
Scopus Author ID 37000312900
ORCID: http://orcid.org/0000-0003-3399-2942
4. Solomadin M.
Researcher ID Web of Science GZB-1407-2022
http://orcid.org/0000-0003-4219-1055
5. Yegorov S.
PhD
ORCID: http://orcid.org/0000-0002-7136-7921
6. Aitpenov T.