Project Manager
Yerlan Dutbayev
Candidate of Agricultural Sciences, Professor
Scopus Author ID: 57191979638
Researcher ID: AAS-6955-2020
ORCID: 0000-0001-8847-091X
About the project
Relevance
As a result of the research will be achieved these goals and results of the project: will be identified sources for resistance to wheat root rot associated with Bipolaris sorokiniana and Fusarium culmorum. Will be used phylogenetic analysis of pathogens based on the study of pathogen isolates using DNA technologies, and morphological and cultural analysis. Will be identified sources of resistance of wheat root rot associated with Bipolaris sorokiniana and Fusarium culmorum, based on the studying of the wheat collection for resistance to collection isolates and populations of the pathogen in laboratory and field conditions in 2 contrast zones of Kazakhstan. Will be selected perspective lines and varieties of wheat with resistance to agents of root rot, which adapted to conditions of Almaty and Aktobe regions.
The purpose of the project
Identifying sources of resistance to wheat root rot caused by Bipolaris sorokiniana and Fusarium culmorum using plant pathological and molecular approaches
Expected and achieved results
The expected results of the research are as follows:
- Sources of resistance to wheat root rot associated with Bipolaris sorokiniana and Fusarium culmorum will be identified.
- Phylogenetic analysis of the pathogens will be conducted based on the study of isolates using DNA technologies and morphological-cultural analysis, confirming their species classification.
- Based on the study of the wheat collection and isolates in laboratory and field conditions in two contrasting zones of Kazakhstan, sources of resistance to wheat root rot associated with Bipolaris sorokiniana and Fusarium culmorum will be identified.
- Promising wheat lines and varieties that are resistant to isolates and populations of root rot and adapted to the conditions of the Almaty and Aktobe regions will be selected.
Achieved results.The planned volume of research scheduled for 2023 has been fully completed. The study of the productivity potential of wheat germplasm and the formation of a wheat sample collection for subsequent study of resistance to root rot have been carried out. As a result of implementing this stage of research, a collection of wheat samples consisting of 80 varieties and lines of wheat has been formed, including 40 samples of spring hard wheat and 40 samples of spring soft wheat. Pre-sowing seed treatment with fungicides was performed, and fungus inoculum was prepared to create a provocative background. The analysis of the crop structure of the wheat collection was conducted in each zone, with 3 replications and on 3 backgrounds. The analysis of the elements of crop structure was carried out according to the methodology of state variety trials for agricultural crops. The main productivity elements were evaluated: plant height, productive tillering, spike length, number of spikelets and grains per spike, grain weight per spike, and 1000 grain weight. Phenological observations were made on wheat crops. Monitoring of disease development was conducted, and infectious material of root rot was collected in the Almaty and Aktobe regions. As a result of this stage of research, the distribution and development level of wheat root rot pathogens in the Almaty and Aktobe regions were determined.
In the next stage of research, we isolated and maintained cultures of root rot fungi. Morphological and cultural characteristics of B. sorokiniana and Fusarium culmorum isolates from the Almaty and Aktobe regions were studied. Fungal cultures were obtained, and morphological and cultural characteristics of 70-80 fungal isolates from 2 regions of Kazakhstan were described. The study revealed that winter wheat seeds germinated on sand showed symptoms of fusarium and root rot. Phytosanitary analysis showed that on average, 91-94% of the seeds were healthy, while 5-6% were infected with common root rot Bipolaris sorokiniana and Fusarium spp. The growth dynamics of different strains of Bipolaris sorokiniana on nutrient media isolated from wheat seeds showed that environmental factors and isolates influenced colony area. On the other hand, the growth dynamics of Fusarium spp. showed that colony area depended on the time of day (P <0.05 ***). Biological characteristics of Fusarium spp. and Bipolaris sorokiniana conidia were described, including length, width, and number of septa.
DNA extraction was carried out from fungal isolates to determine the quality of the product. Fungal identification and species determination were performed. DNA samples from fungal isolates were prepared, and fungi were identified and classified by their species. Molecular analysis of all obtained isolates using the COSA_F/COSA_R primer from affected plant tissues confirmed their identification as B. sorokiniana. Three isolates of this fungus were identified from the roots of winter wheat variety #231, three from the roots of spring wheat variety Kazakhstan 10, and four from the roots of winter wheat variety Steklovidnaya 24 (Kz 60, Kz 69, Kz 70, Kz 78b).
List of publications (with links to them) and patents
Kharipzhanova A., Ismailova E., Dutbayev Y., Sybanbaeva M., Sultanova N. Morphological and cultural characteristics of fungal pathogens of winter wheat seeds in Almaty region //Research, Results. – 2023. – №. 3 (99). https://orcid.org/0000-0002-7329-1449
Members of the research group
Kohmetova Alma
Academic degree, title: Doctor of Biological Sciences, Professor
Scopus Author ID: 9638035500
Researcher ID: M-6618-2015
ORCID: 0000-0002-4141-1784
Tsygankov Vladimir
Academic degree, title: Candidate of Agricultural Sciences, Professor
Scopus Author ID: 35726104000
Researcher ID: GIY-1191-2022
ORCID: 0000-0002-3652-3888
Raimbekova Baktigul
Academic degree, title: Candidate of Agricultural Sciences, Assosiate Professor.
Scopus Author ID: 56127302900
ORCID: 0000-0001-6867-4018
Kharipzhanova Aidana
Academic degree, title: Master of Agricultural Sciences, PhD doctoral student
Scopus Author ID: 57819377000
Researcher ID: AGU-3467- 2022
ORCID: 0000-0002-7329-1449
Keishilov Zhenis
Academic degree, title: Master of Agricultural Sciences
Scopus Author ID: 57221382183
Researcher ID: ABE-9523-2021
ORCID: 0000-0003-2126-6951
Kumarbaeva Madina
Academic degree, title: PhD Doctor of Agricultural Sciences
Scopus Author ID: 57213619570
Researcher ID: ABE-9503-2021
ORCID: 0000-0002-5588-6772
Bakhytuly Kanat
Academic degree, title: Master of Biological Sciences
ORCID: 0000-0001-6084-0480
Tsygankov Artem
ORCID: 0000-0002-1782-962X
Bolatbekova Ardak
Academic degree, title: Master of Biological Sciences
Scopus Author ID: 57226105668