About the project
Relevance
There are 544 hereditary abnormalities in cattle in the world, of which more than 60 genetic abnormalities can be identified by molecular genetic diagnostic methods. For diagnostics of genetic defects: BLAD, CVM, DUMPS, BC, FXID, Brachyspina syndrome, fertility haplotypes in cows HH1, HH3, HH4, HH5, HCD, subfertility syndrome in bulls-producers are used diagnostic methods developed by domestic. However, our country has not developed methods of detection of fertility haplotypes HH2, HH6, in Holstein breed and JH1, JH5 in Jersey breed and accordingly no diagnostic studies are conducted. Within the framework of the project realization methods of diagnostics of haplotypes HH2, HH6, JH1, JH5 with the help of PCR-PDRF analysis and Tetra-Primer ARMS reaction in cows and bulls of producers will be developed. The status of breeding farms on these haplotypes will be determined, a new method of diagnostics, Tetra-Primer ARMS reaction method, which has an advantage over existing methods of detection will be introduced into laboratory practice, the cost of diagnostics will be reduced due to the exclusion of restrictionase application. The realization of this project has an economic effect, as the developed methods allow to control the process of spreading of harmful mutations and reduces embryonic mortality in cows. The project has commercial potential, the sphere of application of the obtained results are dairy farms, breeding centers, companies selling frozen semen of foreign selection.
Objective
Development and modification of molecular-genetic methods of diagnostics of carriers of fertility haplotypes HH2, HH6, JH1, JH5 in cattle, determination of the status of breeding farms on the prevalence of these diseases, introduction into practice of alternative methods of detection of fertility haplotypes (Tetra-Primer ARMS reaction) and reduction of the risk of disease incidence in breeding animals.
