Laboratory of microclonal reproduction of plants

The goal is to create collections, improve health and reproduction in vitro, study, maintain and restore plant biodiversity.
Tasks:
- Training of specialists in the field of biotechnology, breeding, molecular biology.
- Conducting scientific research on bioengineering, plant breeding, preservation of biological resources with the involvement of foreign experts
- Transfer and implementation of innovative technologies and developments, support for the export of agricultural crops.
- Ensuring the need of the agricultural enterprise for certified planting material of fruit and berry, forest, vegetable and other agricultural crops

- Diagnostics of viral diseases of vegetable and fruit crops.          
- Improvement of planting material of vegetable and fruit and berry crops.
- Reproduction in vitro of planting material of vegetable and fruit and berry crops.
- Adaptation (container culture) of the in vitro planting material of vegetable and fruit and berry crops. 
- Development of health improvement and reproduction systems for valuable varieties of any vegetable and fruit and berry crops.
- Cryopreservation of plant collections. 
- Restoration of lost germination collections of plants.

AR09058208 Screening of cultivated and wild forms of the gene pool of leguminous crops for disease resistance to search for source material for breeding.  Head Suleymanova G.A.
AR13068068 "Molecular screening and selection of resistant apple varieties to fungal diseases using DNA marker technologies". Head of Galymbek K.

Water treatment unit
Nutrient Media Preparation Unit  
IFA block
Laminar flow unit
Cryobank
PCR unit

Technical equipment

Laminar-boxes of I and II level, Rotor Gene Q real time amplifier, MRC UTKBS-150LV autoclave, dry-frying cabinets

Daihan SOF-W105, GFL distillers and bidistillators, Evoqua UVUF TM dionizer, Millipore DURAN GROUP, magnetic stirrers Heidolph MR Hei-Standard, Daihan SHO-1Di shaker incubator, Panasonic MPR-715F refrigerators, Panasonic MDF-MU549DH-PE and MPR-514R-PE freezers, Daihan HHP400 climate chambers, BertHold LB920 ZOOM HT HS4m immune enzyme analyzer, BertHold Stat FAX 4300 flushing mixer, Mshot MF52N inverted microscope, Cleaver scientific Omni Doc pro geldoc system, Ohaus FC5718 and Hettich MIKRO 200R centrifuges, Shimadzu UV-1900 spectrophotometer, Agilent 2100 bioanalyzer.

Projects have been submitted to the competition for the GF on scientific and scientific-technical projects for 2023-2025 by the EOM of the Republic of Kazakhstan:

AP19676378 Development of methods for obtaining elite planting material of seed crops based on the study of viral diseases using biotechnology methods.  Hands. Madenova A.K. Requested financing 98556,5 thousand tenge.

AP19679681 Creation of highly productive, pathogen-resistant tetraploid pumpkin rootstocks with evaluation of rootstock-graft combinations with cucumber and melon. Hands. Dzhantasov S.K. The requested financing is 92982.3 thousand tenge. 

On the commercialization of the results of scientific and (or) scientific and technical activities, pitches were prepared and presented for representatives of the Science Foundation on 2 topics, for further participation in the competition for grant financing of projects for the commercialization of scientific results (RNNTD):

"Plantation afforestation "POPULUS"" R&D No. 0195RK00469 in NCGNTE. purpose: Production of poplar planting material for industrial cultivation and landscaping, from 100 thousand to 600 thousand pieces annually. The planned amount is 300 million tenge.
"Production of virus-free planting material of elite raspberry varieties" Research AR 05131764 RNNTD 22RK0021 purpose: Production of planting material with a closed root system of elite raspberry varieties, 300,000 plants annually. The planned amount is 300 million tenge.

The number of publications for 2021-2022 in scientific journals is only 30 articles, including:
1. Articles of KKSON - 4 (2021) and 12 (2022) – a total of 16;
2. In the Scopus database - 5 (2021) and 2 (2022) – a total of 7 (including 2 - Q1 and 1- Q2);
3. Patents of the Republic of Kazakhstan – 4 (2021) and 2 (2022) – a total of 6.      
4. The Hirsch index is 4 employees, the total value of the Hirsch index is 10.

A cooperation agreement has been signed with NETSKI GmbH, Germany on the international project "Sustainable regional management and creation of added value for the preservation of ancient apple varieties in Kazakhstan and Kyrgyzstan "ALMA".  The project has been approved by the German Federal Environment Fund (OV11). The Kazakh-German Institute for the Support of Biological Resources for Biodiversity Conservation has been opened on the basis of the laboratory.
 

Internships
1. Kaldybayeva D., MNS. Master's degree, October 1 - 31, 2022, within the framework of the grant project AP14869380 "Development of technology for obtaining high-quality planting material of stone crops, cured of major viral diseases using a complex of methods of nursery breeding and biotechnology", she completed an internship in the direction of "Identification of fungal diseases of plants using molecular methods" at the University of Pamukkale, Department of Biology, Faculty of Science, Turkey. Dr. Rafika Jayda Beram Cafe. 
2. Madenova A.K., SNC, PhD, December 04-09, 2022. within the framework of the grant project AP14869380 "Development of technology for obtaining high-quality planting material of stone crops, cured of major viral diseases using a complex of methods of nursery breeding and biotechnology" visited the University of Erciyes, Kayseri, Turkey to familiarize themselves with the work and exchange of experience on molecular identification of viral diseases of stone crops. The work was carried out with the project collaborator Dr. Aydin Uzun and the staff of the Department of Fruit and Vegetable Growing. We got acquainted with the selection works of the department; molecular research of the Laboratory of biotechnology. We visited collection and breeding gardens of wild apricot. The issues of methods of improving plants from viral diseases were discussed. Visited the genome laboratory Genome and Stem Cell Center and got acquainted with the works of Dr. Kahraman Gyurkan. We visited the laboratory of clonal micro-propagation of plants. We got acquainted with the activities of the department; primers were obtained; a further collaboration plan was drawn up within the framework of the project. Participated in the seminar of Dr. Uzun on molecular methods in the selection of fruit and vegetable crops at Istanbul University, Istanbul.
3. Irkitbay Azhargul, international scholarship "Bolashak", specialty "Protection and quarantine of plants. Fruit and vegetable farming" Code: 51T. term: 12 months (01.05.2023 – 30.04.2024), subject: Scab (Venturia inaequalis), bacterial burn (Erwinia amylovora) and diseases of summer apple trees. Internship country: USA, host country: University of Illinois, Urbana-Champaign. Department of Plant Science, Professor Mohammad Babadoost.

   Work is being carried out in the laboratory:
Genotypes of poplar, apple, raspberry, pear, lavender, strawberry, grape, black currant, blackberry, kiwi are introduced into culture in vitro. Propagate in vitro: genotypes of poplar, turanga, apple, raspberry, pear, lavender, strawberry, grape, black and red currant, blackberry. 
Work has begun to identify resistant apple samples to scab and powdery mildew. Sampling was carried out, strains of fungal diseases were isolated with their identification.